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KMID : 0368420060490020153
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Journal of Plant Biology
2006 Volume.49 No. 2 p.153 ~ p.159
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Changes in cellular polyamine contents and activities of their biosynthetic enzymes at each phase of the cell cycle in by-2 cells
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Jang Su-Jin
Cho Hyun-Wook
Park Ky-Young
Kim Yong-Bum
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Abstract
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We analyzed changes in polyamine contents and the activities of biosynthetic enzymes during each phase of the cell cycle for a synchronized population of BY-2 cells. Based on our analysis of H3-thymidine incorporation flow cytometry, and the mitotic index, the M and G2 phases seemed to occur at 8 h and from 2.5 to 8 h, respectively, after the release of aphidicolin. The respective activities of arginine decarboxylase (ADC), Ornithine decarboxylase (ODC), and S-adenosyl methionine decarboxylase (SAMDC) at the beginning (7.4, 11.2, and 5.5 nmol mg-1 protein h-1) were increased to 22.6, 22.1, and 15.1 nmol mg-1 protein h-1. However, those increases do not coincide with the general change in polyamines reported from animal cells. In addition, the bi-phasic activation of polyamine biosynthetic enzymes, such as those found in the general animal model, was observed with ADC and ODC but not with SAMDC. These results suggest that the general animal model for explaining polyamine changes and SAMDC activation in the cell cycle cannot be applied to BY-2 cells. Further, our flow-cytometric analysis of cell populations may be a useful tool for evaluating the effects of polyamines on cell cycle progression in BY-2 cells.
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KEYWORD
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BY-2 cells, cell cycle, flow-cytometric analysis, Nicotiana tabacum, polyamines, S-adenosyl methionine decarboxylase
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